AT 280 SPE

The Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) instrument performs automated SPE of large-volume liquid samples for organic analysis. Liquid–liquid extractions that normally take long hours can be automated using a Dionex AutoTrace 280 SPE instrument. Extraction steps that normally require constant attention by chemists and technicians are performed automatically, and a Dionex AutoTrace 280 instrument significantly reduces sample handling compared to techniques such as separatory funnel or vacuum manifold for SPE. The tedious and time-consuming steps of liquid-liquid extraction can be greatly decreased by automating the SPE steps of condition, load, rinse, and elute. The Dionex AutoTrace 280 instrument offers a lower sample preparation cost-per-sample than other techniques by reducing solvent and labor cost by up to 90%.


  • Extractions for liquid sample sizes of 20 mL–20 L
  • Dramatic solvent reduction and reduced sample handling
  • Wide range of applications
  • Automation
  • Approved for use by many government agencies

The Dionex AutoTrace 280 instrument provides solutions for many industries:


  • Pesticides and herbicides
  • PAHs and semi-volatiles
  • PCBs
  • Dioxins and furans
  • Oil and grease
  • Explosives
  • Brominated flame retardants
  • Urons (phenyl urea herbicides)

The Dionex AutoTrace 280 instrument meets requirements for Solid-Phase Extraction for U.S. EPA 500 and 600 Method series.

Pharmaceutical and Natural Products

  • Active ingredients in liquids
  • Extractables in packaged water
  • Extractables in drug delivery devices and packaging

Food and Beverage

  • Vitamins and antibiotics
  • Contaminants in bottled drinks
  • Natural products
  • Pesticides and herbicides in liquids
  • Pesticide residues
  • Herbal and dietary supplements
AutoTrace 280 Solid Phase Extraction Instrument Specifications
Gas Regulator and Gas Gauge Range: Output: 0–30 psi (0–1.4 bars),
Input: 100 psi (6.9 bars) maximum
Net Weight: 150 lbs. (68.1 kg)
Dimensions (h × w × d): 23 × 25 × 27 in (57 × 63.5 × 69 cm)
Sample Rack: 8 × 16.5 × 13 in
500 & 600 Series EPA Methods: 24 methods stored in the AutoTrace instrument,
unlimited number of methods stored in PC
Electrical: Voltage: 100, 120, 220, or 240 V ± 10%
Frequency: 47–63 Hz
Power: 150 Volt AC
Liquid Management: Air Syringe: One 10 mL air syringe
LH Syringe: One 10 mL liquid handling syringe
12-port Valve: Rotary, sliding Rulon® seal
Valves: 3-way, PTFE
Nozzles: Stainless steel
Tubing: PTFE, 1/16" ID, (6 total supplied)
Sample Pumps: Displacement: Positive
Accuracy: ± 2.5%
Tube Fitting: Kynar®
Piston and Liner: Ceramic
Non Use: Not for use with acetic acid and acetone
Solid Phase Extraction Configurations: 1 mL Syringe: Compatible cartridge
3 mL Syringe: Compatible cartridge
6 mL Syringe: Compatible cartridge
47 mm SPE disk
Rack and Collection Vial Sizes Max. Solvent Volumes
15 mL conical tubes 14 mL
11 mm GC vials 1.5 mL
4 mL screw cap vials 3.5 mL
17 x 60 mm vials 7.5 mL
16 x 100 mm test tubes 11 m

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